Culturing Entomophthora muscae Berkeley
Interested in cultivating your own zombie flies? Anyone can do it with persistence and the right tools.
Culturing E. muscae Berkeley in the lab:
Culturing E. muscae Berkeley at home:
Catching Wild Drosophila and E. muscae
I suspect that you can find E. muscae in any temperate environment where host flies are present. This protocol can serve as a good starting point for catching wild fruit flies and, hopefully, E. muscae, in your own backyard.
Drosophila CO2 anesthesia at home
In working with flies in my apartment, I’ve found it helpful to knock them out with CO2 using a SodaStream before transferring them to a chilled aluminum block for sorting.
I used this paraffin-embedding and microtomy protocol to localize E. muscae Berkeley cells in infected flies:
Easy method to sort Drosophila pupae
This transparency hack from Dave Zucker and Tom Alisch makes it easy to sort pupae.