Culturing Entomophthora muscae Berkeley

Interested in cultivating your own zombie flies? Anyone can do it with persistence and the right tools.

Culturing E. muscae Berkeley in the lab:

 

Culturing E. muscae Berkeley at home:

PDF: Maintaining E. muscae in vivo culture in a private residence

Catching Wild Drosophila and E. muscae

I suspect that you can find E. muscae in any temperate environment where host flies are present. This protocol can serve as a good starting point for catching wild fruit flies and, hopefully, E. muscae, in your own backyard.

PDF: Collecting E. muscae-infected fruit flies

Drosophila CO2 anesthesia at home

In working with flies in my apartment, I’ve found it helpful to knock them out with CO2 using a SodaStream before transferring them to a chilled aluminum block for sorting.

PDF: Home anesthesia setup components


Drosophila
histology

I used this paraffin-embedding and microtomy protocol to localize E. muscae Berkeley cells in infected flies:

https://www.protocols.io/view/paraffin-embedding-microtomy-and-fluorescence-in-s-k5ecy3e

Easy method to sort Drosophila pupae

This transparency hack from Dave Zucker and Tom Alisch makes it easy to sort pupae.

https://www.protocols.io/view/video-protocol-for-sorting-drosophila-pupae-bpq3mmyn